Originally presented at the 2015 In Vitro Biology Meeting of the Society for In Vitro Biology
Presented by Brett Gytri. Co-authored by Carolyn Sluis.
Abstract (Original Submission: P-1)
Researchers in universities across the globe have made great strides toward understanding many of the fundamental underlying principals of plant growth regulation, using the model plant Arabidopsis.
This has resulted in a wealth of avenues to explore for better control of plant development and morphology during propagation in vitro. The challenge in the current economic marketplace is to create reliable, consistent, high volume plant production systems using the best research tools and the best scientific breakthroughs, while keeping in mind both the economics and the marketability of the end product.
Experimental design and analysis programs have become increasingly accessible to the tissue culture researcher and can help create multifactorial experimental designs which allow evaluation of many interacting factors in a relatively short time frame simultaneously.
The steps comprising a tissue culture propagation system can be modeled to produce the maximum number of plants while factoring in the best use of labor and materials. The physical parameters of the culture system: the vessels, gas environment and substrate, play a large role in plant quality. Liquid based systems can be compatible with mechanization. Photoautotrophic systems can improve plant quality and normalcy.
Blending the many options into a functional economic propagation system can be challenging. However, most commercial plant tissue culture laboratories subculture their plants in small vessels with gelled, sugar-containing media. Why? We will explore the reasons for this and examine the research tools available for attaining the goal of high volume micropropagation of economically viable crops in novel and sometimes unexpected ways.